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Super-Resolution Imaging in Biomedicine, 1ed
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This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.
About the Author
Alberto Diaspro, Ph.D, is a professor of applied physics and head of the Nanophysics Unit of the Italian Institute of Technology. He also founded the Laboratory for Advanced Microscopy, Bioimaging, and Spectroscopy (LAMBS).
Marc A.M.J. van Zandvoort, Ph.D, is the leader of the Two-Photon Core Facility in Aachen and head of the Advanced Microscopy Unit in the Department of Genetics and Cell Biology at Maastricht University, Netherlands.
Contents
۱. In the Realm of Super-Resolved Fluorescence Microscopy
۲. Super-Resolution in a Standard Microscope. From Fast Fluorescence Imaging to Molecular Diffusion Laws in Live Cells
۳. Photoswitching Fluorophores in Super-Resolution Fluorescence Microscopy
۴. Image Analysis for Single-Molecule Localization Microscopy
۵. Deconvolution of Nanoscopic Images
۶. Re-Scan Confocal Microscopy
۷. Structured Illumination Microscopy
۸. Correlative Nanoscopy. AFM Super-Resolution (STED/STORM)
۹. Stochastic Optical Fluctuation Imaging
۱۰. Super-Resolution Two-Photon Excitation Microscopy Utilizing Transmissive Liquid Crystal Devices
۱۱. Fluorescent Nanodiamonds in Biological and Biomedical Imaging and Sensing
۱۲. Optical Nanoscopy with SOFI
۱۳. Super-Resolution Fluorescence Microscopy of the Nanoscale Organization of RNAP and DNA in E. coli
۱۴. Correlative Live-Cell and Super-Resolution Microscopy and Its Biological Applications
۱۵. SAX Microscopy and Its Application to Imaging of 3D-Cultured Cells
۱۶. Quantitative Super-Resolution Microscopy for Cancer Biology and Medicine
۱۷. Distribution,. Organization, and Dynamics of EGF Receptor in the Plasma Membrane Studied by Super-Resolution Imaging
۱۸. Super-Resolution Microscopy. The Dawn of a New Era in Cardiovascular Research
لینک کوتاه : https://bookbaz.ir/?p=54688
نویسنده : Alberto Diaspro
ناشر : CRC Press; 1 edition
سال انتشار : 2017
زبان کتاب : انگلیسی
نوع فایل : PDF
تعداد صفحات : 448
(ISBN) شابک : 1482244349
قیمت کتاب درآمازون : $242.45
حجم فایل : 21 MB
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