دانلود کتاب درک آنزیم ها: تابع، طراحی، مهندسی و آنالیز
Understanding Enzymes: Function, Design, Engineering, and Analysis, 1ed

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Understanding Enzymes: Function, Design, Engineering, and Analysis focuses on the understanding of enzyme function and optimization gained in the past decade, past enzyme function analysis, enzyme engineering, and growing insights from the simulation work and nanotechnology measurement of enzymes in action in vitro or in silico. The book also presents new insights into the mechanistic function and understanding of enzyme reactions, as well as touching upon structural characteristics, including X-ray and nuclear magnetic resonance (NMR) structural methods. A major focus of the book is enzyme molecules’ dependency on dynamic and biophysical environmental impacts on their function in ensembles as well as single molecules. A wide range of readers, including academics, professionals, PhD and master’s students, industry experts, and chemists, will immensely benefit from this exclusive book.

About the Author

Allan Svendsen did his master of science in biochemistry and protein chemistry in 1985 from the University of Copenhagen, Denmark. From 1986 to 2008, he worked as a research scientist at Nordisk Gentofte A/S and Novo Nordisk A/S. Later, he joined Novo Nordisk A/S and Novozymes A/S as a science manager and then became senior science manager at Novozymes A/S, where since 2008, he has been science director. His research area is protein engineering in general, but he has been working especially with insulin, proteases, oxidases, amylases, and lipases. His work has been within HPLC analysis, purification, downstream processing, and assay development. Lipase protein engineering and structural computer analysis and design of variants have been a central area for around 26 years.

Dr. Svendsen has worked as a coordinator for a lipase protein engineering project at the Nordic Industrial Foundation. He was the organizer and coordinator of an EU-funded nanotechnology project, “Lipid–Lipase Structure Function Relationship: Novel Methods for Studying Interfacial Enzyme Actions and Relation to Structure of Self-Assembled Lipid Nanostructures,” and the co-organizer of another EU-funded nanotechnology project, “BIOSCOPE.” He has supervised many master’s students and a few PhD students and has acted as censor for a number of PhD and master’s theses defenses. He has reviewed manuscripts for various journals, co-edited a special issue of Colloids and Surface B: Biointerfaces (۲۰۰۱), and edited Enzyme Functionality: Design, Engineering and Screening (۲۰۰۲ and 2003 editions) published by Marcel Dekker, USA. He has published more than 100 articles in refereed international journals, has more than 90 patents and patent applications, and has made more than 30 presentations at scientific conferences.

Contents

Chapter 1: A Short Practical Guide to the Quantitative Analysis of Engineered Enzymes
Chapter 2: Protein Conformational Motions: Enzyme Catalysis
Chapter 3: Enzymology Meets Nanotechnology: Single-Molecule Methods for Observing Enzyme Kinetics in Real Time
Chapter 4: Interfacial Enzyme Function Visualized Using Neutron, X-Ray, and Light-Scattering Methods
Chapter 5: Folding Dynamics and Structural Basis of the Enzyme Mechanism of Ubiqurtin C-Terminal Hydroylases
Chapter 6: Stabilization of Enzymes by Metal Binding: Structures of Two Alkalophilic Bacillus Subtilases and Analysis of the Second Metal-Binding Site of the Subtilase Family
Chapter 7: Structure and Functional Roles of Surface Binding Sites in Amylolytic Enzymes
Chapter 8: Interfacial Enzymes and Their Interactions with Surfaces: Molecular Simulation Studies
Chapter 9: Sequencer Structure, Function: What We Learn from Analyzing Protein Families
Chapter 10: Bioinformatic Analysis of Protein Families to Select Function-Related Variable Positions
Chapter 11: Decoding Life Secrets in Sequences by Chemicals
Chapter 12: Role of Tunnels and Gates in Enzymatic Catalysis
Chapter 13: Molecular Descriptors for the Structural Analysis of Enzyme Active Sites
Chapter 14: Hydration Effects on Enzyme Properties in Nonaqueous Media Analyzed by MD Simulations
Chapter 15: Understanding Esterase and Amidase Reaction Specificities by Molecular Modeling
Chapter 16: Toward New Nonnatural TIM-Barrel Enzymes Using Computational Design and Directed Evolution Approaches
Chapter 17: Handling the Numbers Problem in Directed Evolution
Chapter 18: Hints from Nature: Metagenomics in Enzyme Engineering
Chapter 19: A Functional and Structural Assessment of Circularly Permuted Bacillus circulans Xylanase and Candida antarctica Lipase B
Chapter 20: Ancestral Reconstruction of Enzymes
Chapter 21: High-Throughput Screening or Selection Methods for Evolutionary Enzyme Engineering
Chapter 22: Nanoscale Enzyme Screening Technologies
Chapter 23: Computational Enzyme Engineering: Activity Screening Using Quantum Chemistry
Chapter 24: In Silico Screening of Enzyme Variants by Molecular Dynamics Simulation
Chapter 25: Kinetic Stability of Variant Enzymes

قیمت : 3000 تومان
لینک کوتاه : https://bookbaz.ir/?p=50709
نویسنده : Allan Svendsen
ناشر : Pan Stanford
سال انتشار : 2016
زبان کتاب : انگلیسی
نوع فایل : PDF
تعداد صفحات : 884
(ISBN) شابک : 9814669326
قیمت کتاب درآمازون : $204.60
حجم فایل : 17 MB

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۳۰۰۰ تـومان – خرید