دانلود کتاب آنالیز سلولی توسط میکروسکوپ اتمی
Cellular Analysis by Atomic Force Microscopy, 1ed

Despite substantial evidence showing the feasibility of Atomic Force Microscopy (AFM) to identify cells with altered elastic and adhesive properties, the use of this technique as a complementary diagnostic method remains controversial. This book is designed to be a practical textbook that teaches how to assess the mechanical characteristics of living, individual cells by AFM. Following a step-by-step approach, it introduces the methodology of measurements in the case of both determination of elastic properties and quantification of adhesive properties.

In the recent two decades, there has been ample evidence that shows the capability of detecting pathologically changed cells basing on their mechanical properties. Such studies commenced to be attractive for biology and medicine with the development of techniques enabling the measurements at a single-cell level. One of such techniques is atomic force microscopy (AFM). Despite evidence showing the feasibility of AFM to identify cells with altered elastic and adhesive properties, the use of this technique as a complementary diagnostic method, for example, in cancer treatment, raises a lot of controversy. This is primarily due to the still high complexity of the apparatus, the complexity of the experiment and data analysis, and the lack of basis for the rapid verification of the results. In addition, there are also doubts whether a high sensitivity of the method (measuring the properties of a single cell) will be sufficient to validate the cancer cell identification at the same level as it is carried out in the analysis of aspirate composed of millions of cells at different stages of progression.

Contents

۱. Introduction
۲. Cell Structure and Functions
۳. Principles of Atomic Force Microscopy
۴. Quantification of Cellular Elasticity
۵. Adhesive Properties Studied by AFM
۶. Conclusions