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Handbook of Histology Methods for Bone and Cartilage, 1ed

Histotechnology and histomorphometry are the major methodologies in bone and cartila- related research. Handbook of Histology Methods for Bone and Cartilage is an outgrowth of the editors’ own quest for information on bone and cartilage histology and histomorphometry. It is designed to be an experimental guide for personnel who work in the areas of basic and clinical bone and cartilage, orthopedic, or dental research. It is the first inclusive and organized reference book on histological and histomorphometrical techniques on bone and cartilage specimens. The topic has not previously been covered adequately by any existing books in the field. Handbook of Histology Methods for Bone and Cartilage has six major parts and is designed to be concise as well as inclusive, and more practical than theoretical. The text is simple and straightforward. Large numbers of tables, line drawings, and micro- or macro-photographs, are used to help readers better understand the content. Full bibliographies at the end of each chapter guide readers to more detailed information. A book of this length cannot discuss every method for bone and cartilage histology that has been used over the years, but it is hoped that major methods and their applications have been included.

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Contents

۱. Introduction to Experimental Bone and Cartilage Histology
۲. Cell Structure and Biology of Bone and Cartilage
۳. Normal Structure and Function of Bone
۴. Structure and Function of Articular Cartilage
۵. Bone-Labeling Techniques
۶. Human Bone Biopsy
۷. Biopsy Issues for Bone and Cartilage Tumors
۸. Tissue Harvesting and Fixation
۹. Common Fixatives in Hard-Tissue Histology
۱۰. Decalcification of Bone Tissue
۱۱. Principles of Embedding and Common Protocols
۱۲. Infiltration Techniques and Results in Different Types of Resin
۱۳. Histological Techniques for Decalcified Bone and Cartilage
۱۴. Techniques for Sectioning Undecalcified Bone Tissue Using Microtomes
۱۵. Cutting and Grinding Methods for Hard-Tissue Histology
۱۶. Bone Sectioning Using the Exakt System
۱۷. Bone Sectioning Using a Modified Inner Diamond Saw
۱۸. Bone Sectioning Using the Precise 1 Automated Cutting System
۱۹. Bone, Cement, and Metal Implant Interface Preparation with a High-Pressure Water Cutter
۲۰. Basic Staining and Histochemical Techniques and Immunohistochemical Localizations Using Bone Sections
۲۱. Basic Staining Techniques for Cartilage Sections
۲۲. Histochemical and Immunohistochemical Staining of Cartilage Sections
۲۳. Staining Techniques for Plastic-Embedded Specimens
۲۴. In Situ Hybridization of Bone and Cartilage
۲۵. Bone Histomorphometry: Concepts and Common Techniques
۲۶. Histological Analysis of Bone-Implant Interface
۲۷. Histomorphometric Analysis of Bone–Cement and Cement–Metal Interface
۲۸. Histologic Analysis of Bone Healing
۲۹. Histomorphometry of Metabolic Bone Conditions
۳۰. Histology of Articular Cartilage Repair Stefan Nehrer and Myron Spector
۳۱. Histological Analysis of Cartilage Conditions
۳۲. Histological Analysis of Soft-Tissues Biomaterial Interface: Relevance to Dental Implants
۳۳. Pathological Diagnosis of Common Tumors of Bone and Cartilage
۳۴. Methods for Transmission and Scanning Electron Microscopy of Bone and Cartilage
۳۵. SEM Methods for Observation of the Bone-Implant Interface
۳۶. TEM Methods for Observation of the Bone-Implant Interface
۳۷. Radiological Examination of Calcified Tissues with Emphasis on Bone
۳۸. Microangiography, Macrosectioning, and Preparation for Contact Radiography
۳۹. Recognizing and Interpreting Histology Artifacts in Hard-Tissue Research