دانلود کتاب میکروسکوپ فلورسانس پیشرفته: روش ها و پروتکل
Advanced Fluorescence Microscopy: Methods and Protocols, 2015th

This volume provides an overview of advanced  fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,  Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

From the Back Cover

This volume provides an overview of advanced  fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,  Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

Contents

۱ The PhysicalBasis of TotalInternal Reflection Fluorescence (TIRF) Microscopyand Its Cekilar Applications
۲ Two-Photon Excitation Microscopy and Its Applicationsin Neuroscience
۳ Live Spheroid Formation Recorded with LightSheet-BasedFluorescence Microscopy
۴ Fluorescence Microscopy-BasedRNA Interference Screening
۵ Fluorescence Resonance Energy Transfer Microscopy (FRET)
۶ Locating Protein-Protein Interactionsin Living Cells Using Fluorescence Lifetime Imaging Microscopy
۷ Analysis of Biomolecular Dynamicsby FRAP and Computer Simulation
۸ Fluorescence Correlation Spectroscopy
۹ Homo-FRET Imaging Highlights the Nanoscale Organization of Cell Surface Molecules
۱۰ Practical Structured Illumination Microscopy
۱۱ ۴PiMicroscopy of the Nuclear Pore Complex
۱۲ Application of STEDMicroscopy to CeSBiology Questions
۱۳ Three-Dimensional Photoactivated Localization Microscopy with Genetically Expressed Probes
۱۴ Direct Stochastic Optical Reconstruction Microscopy(dSTORM)
۱۵ Optogenetics: Optical Controlofa Photoactivatable Rac in Living Cells