دانلود کتاب ژن کلونینگ و آنالیز DNA: معرفی
Gene Cloning and DNA Analysis: An Introduction, 7ed

Known world-wide as the standard introductory text to this important and exciting area, the seventh edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations.

In addition to a number of informative changes to the text throughout the book, the chapters on DNA sequencing and genome studies have been rewritten to reflect the continuing rapid developments in this area of DNA analysis:

• In depth description of the next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes
• New material on the use of ChiP-seq to locate protein-binding sites
• Extended coverage of the strategies used to assemble genome sequences
• Description of how the Neanderthal genome has been sequenced and what that sequence tells us about interbreeding between Neanderthals and Homo sapiens

Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves.

Contents

PART I The Basic Principles of Gene Cloning and DNA Analysis

Chapter 1: Why Gene Cloning and DNA Analysis are Important
Chapter 2: Vectors for Gene Cloning: Plasmids and Bacteriophages
Chapter 3: Purification of DNA from Living Cells
Chapter 4: Manipulation of Purified DNA
Chapter 5: Introduction of DNA into Living Cells
Chapter 6: Cloning Vectors for Escherichia coli
Chapter 7: Cloning Vectors for Eukaryotes
Chapter 8: How to Obtain a Clone of a Specific Gene
Chapter 9: The Polymerase Chain Reaction

PART II The Applications of Gene Cloning and DNA Analysis in Research

Chapter 10: Sequencing Genes and Genomes
Chapter 11 Studying Gene Expression and Function
Chapter 12: Studying Genomes

PART III The Applications of Gene Cloning and DNA Analysis in Biotechnology

Chapter 13: Production of Protein from Cloned Genes
Chapter 14: Gene Cloning and DNA Analysis in Medicine
Chapter 15: Gene Cloning and DNA Analysis in Agriculture
Chapter 16: Gene Cloning and DNA Analysis in Forensic Science and Archaeology