دانلود کتاب کشت سلولهای حیوانات: راهنمای تکنیک اساسی و برنامه های تخصصی
Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 7ed

Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols.

This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews.  New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.

Contents

۱. Introduction
۲. Biology of Cultured Cells
۳. Laboratory Design and Layout
۴. Equipment and Materials
۵. Aseptic Technique
۶. Safety, Bioethics, and Validation
۷. Culture Vessels and Substrates
۸. Defined Media and Supplements
۹. Serum-Free Media
۱۰. Preparation and Sterilization
۱۱. Primary Culture
۱۲. Subculture and Cell Lines
۱۳. Authentication and Validation
۱۴. Microbial Contamination
۱۵. Cryopreser/ation and Banking
۱۶. Cloning and Selection
۱۷. Cell Sorting
۱۸-. Cell Line Characterization
۱۹. Differentiation
۲۰. Three-Dimensional Culture
۲۱. Scale-up and Automation
۲۲. Senescencer Immortalization, and Transformation
۲۳. Quantitation
۲۴. Cytotoxicity
۲۵. Culture of Specific Cell Types
۲۶. Stem Cells
۲۷. Training Programs
۲۸. Problem Solving
۲۹. In Conclusion